WEBUpon uptake by the cell, citrate is cleaved by citrate lyase to oxaloacetate and acetate. The oxaloacetate is then metabolized to pyruvate and CO2. Further metabolic breakdown is dependent upon the pH of the medium. Under alkaline conditions, pyruvate is metabolized to acetate and formate.
Citrate test - Wikipedia
WEBPrinciple. Bacteria are inoculated on a medium containing sodium citrate and a pH indicator such as bromothymol blue. The medium also contains inorganic ammonium salts, which are utilized as sole source of nitrogen. Use of citrate involves the enzyme citrate lyase, which breaks down citrate to oxaloacetate and acetate.
Simmons Citrate Test: Principle, Procedure, Results, and Applications
WEBAug 1, 2024 · The medium used in this test, Simmons Citrate Agar, contains sodium citrate as the sole carbon source and ammonium dihydrogen phosphate as the sole nitrogen source. The agar also includes bromothymol blue, a pH indicator that shifts color in response to changes in the medium’s pH.
ATP citrate synthase - Wikipedia
WEBATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. In animals, the product, acetyl-CoA, is used in several important biosynthetic pathways, including lipogenesis and cholesterogenesis . [ 5 ]
Structure of ATP citrate lyase and the origin of citrate synthase in ...
WEBApr 3, 2019 · Crystal structures of ATP citrate lyase from bacteria, archaea and humans unravel how the enzyme directs the formation of the central metabolite acetyl-CoA, and shed light onto the evolutionary...
1.20: Citrate Test - Biology LibreTexts
WEBWhen the medium changes color to blue, the test indicates the bacterial species is citrate positive (right). Bacterial species that are capable of metabolizing citrate are considered citrate positive and will cause Simmons' citrate agar to change from green to blue.
ATP Citrate Lyase - an overview | ScienceDirect Topics
WEBATP citrate lyase (ACLY) is a cytosolic homotetrameric enzyme that catalyzes the conversion of citrate and coenzyme A (CoA) to acetyl-CoA and oxaloacetate, with the simultaneous hydrolysis of ATP to ADP and phosphate.
The Citrate Enzymes: Their Structures, Mechanisms, and Biological ...
WEBJan 1, 1972 · The enzymes that catalyze lyase reactions on citrate to yield a C 2 and a C 4 unit are: citrate lyase, citrate synthase, and adenosine triphosphate (ATP) citrate lyase. Citrate lyase has been reported only in certain bacteria, citrate synthase has been found in all cells examined for it, and ATP citrate lyase has been found only in eukaryotic ...
ATP-citrate lyase: a mini-review - PubMed
WEBMay 25, 2012 · ATP-citrate lyase (ACLY) is a cytosolic enzyme that catalyzes generation of acetyl-CoA from citrate. Acetyl-CoA is a vital building block for the endogenous biosynthesis of fatty acids and cholesterol and is involved …
Allosteric role of the citrate synthase homology domain of ATP citrate …
WEBApr 19, 2023 · ATP citrate lyase (ACLY) is a major source of nucleocytosolic acetyl-CoA, an essential building block for the biosynthesis of fatty acids, isoprenoids, and cholesterol, as well as a cofactor...